Brown suffered the injury in the divisional round. The Bucs were hopeful he could play in the conference championship game against the Green Bay Packers, but the veteran was ruled out after not practicing.
Scotty Miller flourished in an increased role against the Packers while catching two passes, including a 39-yard touchdown toss from Tom Brady before halftime.
Brown posted 45 receptions for 483 yards and four touchdowns during the regular season after making his Buccaneers debut in Week 9.
The Buccaneers have won seven straight games and will challenge the Kansas City Chiefs in Super Bowl LV on Feb. 7.